Device

Part:BBa_K2548012:Design

Designed by: Zhuoyi Yang   Group: iGEM18_BNDS_CHINA   (2018-10-01)
Revision as of 14:14, 16 October 2018 by Sallina yxl0709 (Talk | contribs)

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LacI - pTac - SpALF4 + 6xHis Tag - Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides with a broad spectrum of antimicrobial activity. SpALF4 is an ALF homolog identified from Scylla paramamosain (Mud crab) that has shown antimicrobial activity against A. hydrophila. Our aim is to obtain the cDNA sequence of SpALF4 from Scylla paramamosain and insert it into the genetic expression of E. coli to express large amount of SpALF4 to treat A. hydrophila invasions.


Source

Zhu, L., Lan, J., Huang, Y., Zhang, C., Zhou, J., Fang, W., Li, X. (2014). SpALF4: A newly identified anti-lipopolysaccharide factor from the mud crab Scylla paramamosain with broad-spectrum antimicrobial activity. Fish & Shellfish Immunology,36(1), 172-180. doi:10.1016/j.fsi.2013.10.023

References