Device

Part:BBa_K2548012

Designed by: Zhuoyi Yang   Group: iGEM18_BNDS_CHINA   (2018-10-01)
Revision as of 14:13, 16 October 2018 by Sallina yxl0709 (Talk | contribs)


LacI - pTac - SpALF4 + 6xHis Tag - Terminator

This design is used to synthesize and extract distinct SpALF4 in order to test its effectiveness. We chose the pTac promoter, a hybrid of Lac and Trp promoters, to induce SpALF4 production in E. coli because it is the most accessible promoter in our lab. The promoter can be induced by IPTG to remove the repression on transcription from LacI. We also add 6xHis-tag after sequences of SpALF4 to extract it out with the nickel column.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None