Composite

Part:BBa_K2587030:Design

Designed by: Sarah Seyffert   Group: iGEM18_Duesseldorf   (2018-10-04)
Revision as of 14:08, 16 October 2018 by SaSey (Talk | contribs) (→‎Source)

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Pj23106_RBS_ddh_T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 422
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 390
    Illegal NgoMIV site found at 615
    Illegal AgeI site found at 825
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We performed a side directed mutagenesis of the recognition sites of the restriction enzymes BsaI and BbsI in the coding sequence of ddh.

Source

Ddh was isolated from the genome of Corynebacterium glutamicum. The other parts were taken from the CIDAR MoClo toolbox

References