Part:BBa_K2812001

Coding sequence for trunctated Lysostaphin

The biobrick contains the coding domain for truncated lysostaphin. Lysostaphin targets the cell wall peptidoglycan found in certain Staphylococci by cleaving its cross-linking pentaglycine bridges. Among others, it is effective for degrading Staphylocuccus aureus biofilms.¹ TU Eindhoven 2018 used lysostaphin to kill S. aureus for preventing wound infections.

The encoding part of the lysostaphin has been derived from BBa_K748002, made by iGEM Harbin 2012 and is also used by iGEM Stockholm 2016. iGEM Eindhoven 2018 codon optimized this lysostaphin construct.

Usage and Biology

Lysostaphin

Lysostaphin is an antimicrobial agent produced by Staphylococcus simulans. Lysostaphin belongs to the major class of antimicrobial proteins and peptides known as bacteriocins. Bacteriocins are proteins or peptides produced by bacteria, displaying a bactericidal activity against other subpopulations of bacteria. S. simulans has a protective immune system against lysostaphin, protecting itself from autolysis by its own produced proteins.²

Through cell-wall degradation of the target, lysostaphin has the ability to kill several Staphylococci strains. It owes its cell-wall degradation activity to the endopeptidase activity on pentaglycine cross-bridges in the peptidoglycan layer. Specific cleavage between the third and fourth glycine residue leads to the lysis of the peptidoglycan layer and death of the bacteria. Resistance to lysostaphin can occur however, due to a higher amount of serine residues instead of glycines in the peptidoglycan layer.³

Sources

1) Tossavainen, H., Raulinaitis, V., Kauppinen, L., Pentikäinen, U., Maaheimo, H., & Permi, P. (2018). Structural and Functional Insights Into Lysostaphin–Substrate Interaction. Front Mol Biosci.

2) Bastos, M. d., Coutinho, B. G., & Coelho, M. L. (2010). Lysostaphin: A Staphylococcal Bacteriolysin with Potential Clinical Applications. Pharmaceuticals (Basel) , 1139–1161.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 126
  
• 1000
  COMPATIBLE WITH RFC[1000]