Composite

Part:BBa_K2587020:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-17)
Revision as of 13:54, 16 October 2018 by SaSey (Talk | contribs) (→‎Source)

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Plas_RBS_gfp_T


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 131
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 131
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 124
    Illegal BsaI.rc site found at 809


Design Notes

The part is optimised for the use with the CIDAR MoClo Toolbox.


Source

The pLas sequence is from Genbank and codon optimised for E. coli. The other parts are from the CIDAR MoClo toolbox.

References