Composite

Part:BBa_K2587012:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-09-16)
Revision as of 13:42, 16 October 2018 by SaSey (Talk | contribs) (→‎Source)

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P_RBS_luxR_T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 70
    Illegal BsaI.rc site found at 701


Design Notes

The construct is optimised for the use with the CIDAR MoClo and contains Type II S restriction sites.


Source

The sequence for LuxR is obtained from GenBank and codon optimised for E. coli. The remaining parts are from the CIDAR MoClo toolbox.

References