Composite

Part:BBa_K2549061:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-13)
Revision as of 13:40, 16 October 2018 by JennyDu (Talk | contribs) (→‎Source)


CMV-CD8α-V5-LaG16-mN1ce-tTA-P2A-EGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3156
    Illegal XbaI site found at 2154
    Illegal PstI site found at 924
    Illegal PstI site found at 968
    Illegal PstI site found at 1287
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3156
    Illegal NheI site found at 531
    Illegal PstI site found at 924
    Illegal PstI site found at 968
    Illegal PstI site found at 1287
    Illegal NotI site found at 2085
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3156
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3156
    Illegal XbaI site found at 2154
    Illegal PstI site found at 924
    Illegal PstI site found at 968
    Illegal PstI site found at 1287
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3156
    Illegal XbaI site found at 2154
    Illegal PstI site found at 924
    Illegal PstI site found at 968
    Illegal PstI site found at 1287
    Illegal NgoMIV site found at 723
    Illegal NgoMIV site found at 1428
    Illegal NgoMIV site found at 1605
    Illegal NgoMIV site found at 2143
    Illegal NgoMIV site found at 3198
    Illegal AgeI site found at 540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1764


Source

IDT (gBlock).

References