Composite

Part:BBa_K2582008

Designed by: Lo Ho Sing   Group: iGEM18_Hong_Kong-CUHK   (2018-10-07)
Revision as of 13:28, 16 October 2018 by Frederickk (Talk | contribs)

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T7 Promoter-RAP(H7-474-495)-T7 Terminator

This part is the T7 RNA Polymerase-based expression cassette of the RNA Aptamer Probe (RAP) BBa_K2582007. It targets the influenza Hemagglutinin subtype 7 (Gene accession number: CY235363.1) by nucleotide 474-495.

Usage and Biology

This part is assembled for the expression of a RNA Aptamer Probe, a RNA aptamer that enhances the fluorescence of its docking fluorogen upon binding of a complementary RNA. When transformed into any DE3 strains of E. coli, it can be expressed by IPTG induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None