RNA

Part:BBa_K2582005:Design

Designed by: Lo Ho Sing   Group: iGEM18_Hong_Kong-CUHK   (2018-10-07)
Revision as of 12:54, 16 October 2018 by Frederickk (Talk | contribs)


RNA Aptamer Probe for N9 (545-566)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

truncated miniSpinach: From Ong et. al. 2017.

Complementary Probe: Using invitrogen BLOCK-iT RNAi Designer


Source

Synthesized

References

W. Q. Ong, Y. R. Citron, S. Sekine, and B. Huang, “Live Cell imaging of endogenous mRNA Using RNA-Based fluorescence ‘turn-on’ probe,” ACS Chem. Biol., vol. 12, no. 1, pp. 200–205, 2017.