Coding

Part:BBa_K2717008:Design

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)
Revision as of 11:57, 16 October 2018 by MengYunzhu (Talk | contribs) (→‎Design Notes)


GFP-TGATG-mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1425
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

In this composited part, we link GFP with mCherry by using sequence "TGATG" and the whole coding is controlled by Promoter lacI and RBS B0034. We design this part to test the efficiency of start-stop codon "TGATG".

Source

TGATG-EXP-2

References