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Part:BBa_K2560011:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-08-21)
Revision as of 10:57, 16 October 2018 by Henselt (Talk | contribs)


Phytobrick version of 5'Connector Dummy


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The sequence was synthesized in silico by Daniel Stukenberg and ordered from Integrated DNA Technologies(IDT).

Source

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward Oligo: CTCGAACAGAATTCGCGGCCGCTTCTAGAGGGAG Reverse Oligo: CTCACTCCCTCTAGAAGCGGCCGCGAATTCTGTT


References