Coding

Part:BBa_K2812004:Design

Designed by: Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls   Group: iGEM18_TU-Eindhoven   (2018-10-03)
Revision as of 08:45, 16 October 2018 by GuidoOerlemans (Talk | contribs) (→‎Source)


Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1351
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Total DNA sequence has been codon optimized for E. coli.


Source

The designed sequence was synthesized de novo by IDT.

References

1) https://www.uniprot.org/uniprot/Q1R2T5#sequences