Coding

Part:BBa_K2599007

Designed by: YEN-LING CHEN   Group: iGEM18_NCTU_Formosa   (2018-09-16)
Revision as of 07:54, 16 October 2018 by Yen-ling (Talk | contribs)


Antimicrobial peptide - Durancin TW-49M

This biobrick is the basic part of the Durancin TW-49M, more information please look for the composite part (BBa_K2599015).



Figure 1. Basic part of Durancin TW-49M


Introduction

Durancin TW-49M produced by Enterococcus durans QU 49, is a nonpediocin-like class II bacteriocin without modified amino acid. Enterococcus are highly competitive due to their resistance to wide range of pH and temperature. Therefore, the bacteriocins they produce shows their wide range effectiveness on pathogenic bacteria.

Although there is an intramolecular disulfide bond in durancin TW-49M, it is not crucial for activity, while important for the stability of activity.


Mechanism of Durancin TW-49M

The bacteriocins inhibit their target organisms through pore formation. Though the mechanism of each inhibition is vary from species to species, the general process is conserved. To see more details, please search for our project page.


Features of Durancin TW-49M

1. Species Specific

Bacteriocins are antimicrobial peptides that will kill or inhibit bcterial strains closely related or non-related to produced bacteria, but will not harm the bacteria themselves by specific immunity proteins. The organisims that Lacticin Z targets including Enterococcus faecalis, Bacillus subtilis, Bacillus coagulans, etc. More target organisms can be found on [http://bactibase.hammamilab.org/BAC159 bactibase].

2. Eco-friendly

Since Durancin TW-49M is a polypeptide naturally produced by bacteria itself and can inhibit other bacteria without much environment impact. It don't pose threat to other organisms like farm animals or humans. Therefore, this toxin will not cause safety problem.

3. Biodegradable

Durancin TW-49M is a short peptide that will degrade in a short time. After degradation, this antibacterial peptide is harmless to our environment.

Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Durancin TW-49M sequence length is around 213 b.p.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

1. Hanchi, H., et al. (2018). "The Genus Enterococcus: Between Probiotic Potential and Safety Concerns—An Update." 9(1791).

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