Composite

Part:BBa_K2764001

Designed by: CHU Ora-In   Group: iGEM18_Hong_Kong_HKUST   (2018-10-03)
Revision as of 06:20, 16 October 2018 by Yhlauah (Talk | contribs)


Alkane channel protein with BBa_J23104 Constitutive promoter Usage and biology As shown in literature, the AlkL protein may be toxic and inhibit the growth of its host. We decided to test the severity of this toxicity and compare by using Anderson promoters of varying strength. BBa_K2764001 utilizes the promoter BBa_J23104, which gives the biobrick a promoter strength of 0.7 on the Anderson promoter scale. This will be used to measure the effect of toxicity of AlkL based on the growth curve of bacteria transformed with this biobrick.

Growth curve <img src="T--Hong_Kong_HKUST--BBa_001growthrate.jpeg" class="rounded mx-auto d-block" alt="..." width="500px" height="500px">

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 712
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 364
    Illegal NgoMIV site found at 577
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None