Regulatory

Part:BBa_K2596004

Designed by: Priya Aggarwal, Stephanie Budhan   Group: iGEM18_Stony_Brook   (2018-10-09)
Revision as of 04:38, 16 October 2018 by Kledalla (Talk | contribs)


PidiA from Synechococcus elongatus PCC 7942

This is a ferrous-ion repressible promoter for cyanobacteria Synechococcus elongatus PCC 7942.

Usage and Biology

PidiA is a ferrous-ion repressible promoter native to cyanobacteria. The source of the PidiA DNA is a close relative to our chassis (S. elongatus PCC 7942). The promoter features a palindromic repeat which is suggested to be activated by the binding of idiB, a gene immediately downstream of the idiA gene, in a ferrous deficient environment [3].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Our BioBrick has the 77bp full minimal idiA promoter sequence, plus an upstream AT-rich region [3]. We did not include downstream base pairs due to ambiguity in the true start codon [3].


[edit]
Categories
//chassis/prokaryote/cyanobacterium
//promoter
Parameters
biologySynechococcus elongatus PCC 7942
device_typeRepressible Promoter