Regulatory

Part:BBa_K2596003

Designed by: Priya Aggarwal, Lin Yu, Natalie Lo   Group: iGEM18_Stony_Brook   (2018-10-09)
Revision as of 04:17, 16 October 2018 by Kledalla (Talk | contribs)


PpsbA2 from Synechococcus elongatus PCC 7942

This a high-light inducible promoter from cyanobacteria Synechococcus elongatus PCC 7942

Usage and Biology

PpsbA2 is a light-inducible promoter native to cyanobacteria. Specifically, it comes from a strain (Synechococcus elongatus PCC 7942) closely related to our chassis organism. The promoter is activated by high light intensity or blue light, and deactivated by low light intensity or red light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

The promoter contains a conserved AT-box motif just upstream of the ribosome binding site that serves as the binding site for light-activated proteins. For the Biobrick, we kept most of the original promoter, including a crucial upstream element and the core promoter sequence. Our final Biobrick is 157 bp in length.


[edit]
Categories
//chassis/prokaryote/cyanobacterium
//promoter
Parameters
biologySynechococcus elongatus PCC 7942
device_typeInducible Promoter