Regulatory

Part:BBa_K2596003

Designed by: Priya Aggarwal, Lin Yu, Natalie Lo   Group: iGEM18_Stony_Brook   (2018-10-09)
Revision as of 04:14, 16 October 2018 by Kledalla (Talk | contribs)


PpsbA2 from Synechococcus elongatus PCC 7942

This a high-light inducible promoter from cyanobacteria Synechococcus elongatus PCC 7942

Usage and Biology

PpsbA2 is a light-inducible promoter native to cyanobacteria [6]. Specifically, it comes from a strain (Synechococcus elongatus PCC 7942) closely related to our chassis organism [7]. The promoter is activated by high light intensity or blue light, and deactivated by low light intensity or red light [8].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

For the Biobrick, we kept most of the original promoter, including a crucial upstream element and the core promoter sequence. Our final Biobrick is 157 bp in length. The promoter contains a conserved AT-box motif just upstream of the ribosome binding site that serves as the binding site for light-activated proteins [9].


[edit]
Categories
//chassis/prokaryote/cyanobacterium
//promoter
Parameters
biologySynechococcus elongatus PCC 7942
device_typeInducible Promoter