Coding

Part:BBa_K2796028

Designed by: Shuangshuang Pu   Group: iGEM18_LZU-CHINA   (2018-10-04)
Revision as of 02:15, 16 October 2018 by Zhoutuoyu (Talk | contribs)


Exosome booster

  • Considering that TIL cells can't secret enough exosomes under normal condition, we fuse three genes to increase the number of exosomes (Alenquer, 2015), thus increasing the transfer efficiency of exosomes.
  • Exosome booster refers to hSDC4-T2A-STEAP3-P2A-NadB.We identified STEAP3 (involved in exosomebiogenesis), syndecan-4(SDC4; supports budding of endosomal membranes to form multivesicular bodies), and a fragment of L-aspartate oxidase (NadB; possibly boosts cellular metabolism by tuning up the citric acid cycle) as potential synthetic exosome production boosters. Combined expression of these genes significantly increased exosome production.(Ryosuke Kojima et al. 2008)

    • Here is the final device that we designed for this project.

    2018 LZU-CHINA vesicle attacker.png

    It combines with exosome booster and miR attacker under control of induced promoter.More details and description you can obtain in our wiki!

    T--LZU-CHINA--design1.png

    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      INCOMPATIBLE WITH RFC[21]
      Illegal BglII site found at 192
      Illegal BglII site found at 1777
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal AgeI site found at 2657
      Illegal AgeI site found at 3277
    • 1000
      INCOMPATIBLE WITH RFC[1000]
      Illegal SapI site found at 1367


[edit]
Categories
//awards/composite_part/winner
Parameters
None