Composite

Part:BBa_K2680261:Design

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)
Revision as of 23:51, 15 October 2018 by Ajoliver (Talk | contribs) (→‎Design Notes)

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3G sfYFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 783
    Illegal SapI.rc site found at 70


Design Notes

Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

To do

References