Composite
Part:BBa_K2758000:Design
Designed by: Marry Xuan Group: iGEM18_ColegioFDR_Peru (2018-10-10)
Mercury activated Killer Red Protein suicide switch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 237
Illegal BsaI.rc site found at 528
Design Notes
This part is designed to work with another part that consists of a Hg accumulator. This part has been designed to express the Killer Red protein later than the accumulator is expressed from the other construct. In order to delay the expression of Killer Red protein relative to the accumulator, we inserted an RBS with moderate binding capabilities into the K2758000 construct. We built this construct through IDT adding the NotI restriction sites.
Source
This sequence was constructed entirely with Biobricks from the iGEM registry. The sequences are: BBa_K346002 (mercury responsive promotor), BBa_B0030 (RBS), BBa_K1184000 (Killer Red), BBa_B0015 (double terminator).