Regulatory

Part:BBa_K2610008

Designed by: Charlotte de Ceuninck van Capelle, Marjolein Crooijmans   Group: iGEM18_Leiden   (2018-10-09)
Revision as of 22:02, 15 October 2018 by Chardeceuninck (Talk | contribs)


pMreB

MreB, a homologue of actin, is a protein which is expressed in nearly all rod-shaped bacterial cells. It is one of the three types of actin-like filaments present in bacteria. MreB is important for maintenance of cell width and cell viability as it recruits the machinery responsible for the establishment of peptidoglycan, an essential building block for the cell wall. [http://jcs.biologists.org/content/128/11/2009 (Gunning et al, 2015)]

Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.


T--Leiden--heatmap.png
Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the MreB promoter is only slightly activated by nalidixic acid and tetracycline

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None