Part:BBa_K2839000
TALEsp1-Pupsp1 stabilized promoter
TALE1 stabilized promoter driving sfGFP expression
Talesp1 pupsp1 Stabilized Device
Contents:
Short Description
Biology and Functionality
Usage in our Project
Illegal Sites Removal
Cloning Strategy
Results, Characterization
Potential Applications
References
1.Short description:
This part consists of a TAL Effector (TALEsp1), a TALEsp1-stabilized promoter (pupsp1) and a fluorescent marker (sfGFP). This part was originally designed by Thomas Hale Segall-Shapiro (Shapiro et al) and iGEM Thessaloniki modified it to exclude 2 PstI recognition sites, thus making it RFC[10] compatible .It can be used to achieve stabilised expression of the sfGFP marker, decoupled from gene/plasmid copy number.
Basic Part Name Registry link Type Function L2S2P21 BBa_K1401006 Terminator Terminates Transcription PT7A1w2 BBa_K2839021 Promoter Transcription Initiation SarJ BBa_K2839022 Insulator
RBSsp1
BBa_K2753036
RBS
Recognized by ribosome and initiates translation
TALEsp1
BBa_K2839027
Coding Sequence
Binds to the PUPsp1 promoter and inhibits RNAP binding
ECK-12 0029600
BBa_K2486006
Terminator
Terminates Transcription
PUPsp1
BBa_K2839025
Promoter
Transcription Initiation
RiboJ
BBa_K1679042
Insulator
B0032
BBa_B0032
RBS
Ribosome Binding Site
sfGFP
BBa_K1893032
Coding Sequence
Fluorescence Marker
B0015
BBa_B0015
Terminator
Terminates Transcription
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2734
Illegal XhoI site found at 3677 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1707
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 23
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