Part:BBa_K2656015
E protein from Escherichia phage phiX174 Coding Sequence
Part BBa_K2656015 is the Lysis gene from the enterobacteria phage phiX174 coding sequence. The lysis gene E of coliphage PhiX174 encodes a 91-amino acid (aa) residue protein of approximately 10 kDa that mediates inhibition of peptidoglycan biosynthesis of Gram-negative bacteria membrane (1)
This basic part has been sequence adapted to be both compatible with the Biobrick and the [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods.
Thus, it can be combined with other Golden Gate standardized basic parts, such as the ones from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection], to assemble transcriptional units in a BsaI one-pot reaction following the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].
Reference
Bernhardt, T. G., Roof, W. D., & Young, R. (2000). Genetic evidence that the bacteriophage φX174 lysis protein inhibits cell wall synthesis. Proceedings of the National Academy of Sciences of the United States of America, 97(8), 4297–4302.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |