Tag
His-Tag

Part:BBa_K2834002

Designed by: Viana Isabel Pérez Domínguez   Group: iGEM18_Tec-Chihuahua   (2018-10-07)
Revision as of 16:16, 15 October 2018 by Ana Laura (Talk | contribs)

(diff) ↠Older revision | Latest revision (diff) | Newer revision → (diff)


6x His-Tag

Tags are needed in order to purify a recombinant protein, 6x His-tag is one of them. The His-tagged protein can be easily detected and purified using immobilized metal-affinity chromatography (IMAC).


Basic part used in BBa_K2834003, BBa_K2834005, and BBa_K2834006

References

Bornhorst, J. A., & Falke, J. J. (2000). [16] Purification of Proteins Using Polyhistidine Affinity Tags. Methods in Enzymology, 326, 245–254.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None