Coding

Part:BBa_K2308011

Designed by: Yunpeng Dai   Group: iGEM17_ECUST   (2017-10-11)
Revision as of 11:45, 15 October 2018 by MTarek (Talk | contribs)


Amp resistance gene coding sequence

This part is Amp resistance gene coding sequence used in Rhodobacter Sphaeroides.

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] managed to improve AmpR at # BBa_K2534007 by fusing AmpR to AmpR promoter which was found to be RhoN and constitutively essential for plasmid selection in lentiviral delivery transfer plasmids.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 716


[edit]
Categories
Parameters
None