Part:BBa_K2559002
--NOTOC-- Bacterial cellulose synthase X(BglX)
The BBa_K2559001 coding a β-glucosidase, which is occasionally encoded in the bcs operons.
Usage and Biology
BGLX is a β-glucosidase which participates the cyanoamino acid metabolism (ko00460), starch and sucrose metabolism (ko00500), phenylpropanoid biosynthesis (ko00940), metabolic pathways (ko01100), biosynthesis of secondary metabolites (ko01110). The enzyme is expected to participate in hydrolysis of bacterial cellulose, instead of synthesis. Its role in cellulose biosynthesis remains to be further explored.
We transfered bcsZ, H, A, B, C, D, bglX from the Acetobacter xylinum which involve in the process of bacterial cellulose synthesis into the model organism cyanobacteria (Synechocystis sp.pcc6803),and we obtain the transgenic cyanobacteria. Transgenic cyanobacteria with bcsZH-ABCD-bglX genes and the cellulose measurement Cyanobacteria glucose content measurement (3 repeats). The same of quality of transferred cyanobacteria with bcsZH-ABCD-bglX gene and wildtype are treated with lysozyme to break out the cell. The glucose represents the content of cellulose. The addition of cellulase can digest the cellulose to glucose then the bacterial cellulose can measured. The distinct differences between the red column and blue column indicate the high expression intensity of bacteria cellulose. Wilcoxon test indicates our data is reliable.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 516
Illegal XhoI site found at 1206 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2099
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 339
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