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Part:BBa_K515107:Experience
User Reviews
SCAU-China |
The main goal of this characterization is to find out which pH value is the most effective among the 6 pH values in order to obtain a suitable culture condition for the expression of Dendar2.We further found that the part which expressed the protein Dendar2 worked well in E.coli strain DH10B, like in the DH5 alpha. Experiment To figure out the best cultural pH for protein expression, we have tested 6 different sets of pH that including: 4.5, 5.8, 6.2, 7.2, 8.2, 9.1, We also performed a time-lapse culture for 360 mins to find out the best time point for harvesting DH10B that expresses Dendar2.
Every 20 mins, we collected 10 μl bacterial culture and measure the green fluorescence and the red fluorescence before and after photoconversion of Dendar2 by using photon stimulation under UV light. ImageJ was used to analysize the florescent intensity of Dendar2 fluorescence. Result and Findings 1.The acid condition is suitable for the expression of Dendar2 When pH =4.5, the part can have a better working condition . 2.There red fluorescence we observed after photoconversion have stronger intensity than the green fluorescence before photoconversion. 3.Compared with the characterisation show before in the DH5α stain, we made conclusion the expression of Dendar2 can be well in those two E. coli strain.| |