Coding

Part:BBa_K2656015

Designed by: Adrian Requena Gutierrez, Carolina Ropero   Group: iGEM18_Valencia_UPV   (2018-09-23)
Revision as of 08:28, 15 October 2018 by Carolinarp (Talk | contribs)


E protein from Escherichia phage phiX174 Coding Sequence


Part BBa_K2656015 is the Lysis gene from the enterobacteria phage phiX174 coding sequence. The lysis protein E acts inducing host cell lysis in the Enterobacteria phage phiX174 infection. Thus, this sequence induces E.coli cell lysis when expressed. This sequence has been adapted to ve both compatible with the Biobrick and the [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods.

It can be combined with other Golden Gate standardized basic parts, such as the ones from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection], to assemble a transcriptional unit in a one-pot reaction following the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None