Composite

Part:BBa_K2558212:Design

Designed by: Tsinghua 2018   Group: iGEM18_Tsinghua   (2018-10-05)
Revision as of 06:41, 15 October 2018 by MairZhou (Talk | contribs)


NOTOC__ lux pR-HS test device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1074
    Illegal NheI site found at 1097
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 177


Design Notes

With the hope to find an optimal promotor for our NEON system, we designed 9 mutations on sites 18, 19 and 20 near the luxR binding site and tested one on -10 site that TUST 2017 concluded to show decreased leakage. We conducted experiments to evaluate these lux pR mutants’ reaction to AHL stimulation and their leakage level. This test device we designed includes a constantly expressed luxR and a lux pR mutant (like BBa_K2558212 with lux pR-HS promotor https://parts.igem.org/Part:BBa_K2558212).


Source

References

Koch B et al. The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors. Microbiology. 2005 Nov;151(Pt 11):3589-602.