Composite

Part:BBa_K2739007

Designed by: Lok In Lo   Group: iGEM18_Edinburgh_OG   (2018-10-08)
Revision as of 23:31, 14 October 2018 by Owen-lo 923 (Talk | contribs)


LacP-RBS-PhaP-HlyA-Terminator

This composite part is improved from the composite part of BBa_K390501, LacP-RBS-PhaP-HlyA-Terminator, which the Phasin part sequencing result revealed a stop codon. This improved composite part has the stop condon removed.


Usage and Biology

T--Edinburgh_OG_BBa_K2739007--image_1.jpeg

Figure 1. Agarose gel electrophoresis of Lac promoter-Phasin-HlyA product. A. PCR product of Lac promoter-Phasin-HlyA with stop codon removal. B. Enzyme digestion of Lac promoter-Phasin-HlyA PCR product for stop codon removal (lane 1: 1 kb DNA ladder; 2: EcoR1 and HindIII double digestion) with the label of HlyA + pSB1C3 Backbone and Phasin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None