Composite

Part:BBa_K2739007:Design

Designed by: Lok In Lo   Group: iGEM18_Edinburgh_OG   (2018-10-08)
Revision as of 23:02, 14 October 2018 by Owen-lo 923 (Talk | contribs)


LacP-RBS-PhaP-HlyA-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Available Phasin biobrick (BBa_K208001) are found in Lac promoter-Phasin-HlyA biobrick, BBa_K390501. Through sequencing, it was found to contain a stop codon. A set of PCR primers was designed and used to remove the stop codon. In the primer design, a HindIII restriction enzyme site, which is not present in the original biobrick, was introduced and allow the confirmation of the success stop codon removal.


Source The phasin basic part and sequence information were obtained from the Composite part of BBa_K390501.

References

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