Regulatory

Part:BBa_K091117

Designed by: Erin Feeney   Group: iGEM08_Davidson-Missouri_Western   (2008-06-26)
Revision as of 20:01, 26 June 2008 by ErFeeney (Talk | contribs)

pLas promoter

This promoter contains the regulatory region upstream of the LasI gene in Pseudomonas aeruginosa. K091117 includes a LasR binding site as well as a -10 region modified from CATAAA to TATAAA to reflect our pLasXOR promoter design. Activation is expected to occur in the presence of the autoinducer PAI-1 and the LasR protein.

pLas' serves as a control for the evaluation of the pLasXOR promoter, part BBa_K091118.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//function/cellsignalling/LasR
Parameters
control
directionForward
negative_regulators
o_h
o_l
positive_regulators1