Composite

Part:BBa_K2582002

Designed by: Lo Ho Sing   Group: iGEM18_Hong_Kong-CUHK   (2018-10-01)
Revision as of 16:35, 14 October 2018 by Frederickk (Talk | contribs)


T7 Promoter-RAP(N9-545-562)-T7 Terminator

This part is the T7 RNA Polymerase-based expression cassette of the RNA Aptamer Probe (RAP) BBa_K2582001. It targets the influenza Neuraminidase subtype 9 (Gene accession number: CY235364.1) by nucleotide 545-566.

Usage and Biology

This part is modified from the Spinach aptamer, the RNA molecule that can bind and increase the quantum yield of

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 90
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None