Coding

Part:BBa_K2729004

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-29)
Revision as of 14:42, 14 October 2018 by MiyamotoKotaro (Talk | contribs)


DENV3 E (Envelope Protein | Dengue Virus Serotype III)

Flavivirus Envelope (E) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1307
    Illegal XhoI site found at 868
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 250
    Illegal SapI.rc site found at 1078


Chactarization

The DENV E protein is a class II fusion protein, essential for attachment, membrane fusion, and assembly.A series of conformational changes occurring in the DENV E protein driven by the endosomal low-pH gives place to the fusion of the viral and endosomal membranes.

Reference

- Nemésio H, Palomares-Jerez F, Villalaín J. The membrane-active regions of the dengue virus proteins C and E. Biochim Biophys Acta. 2011 Oct;1808(10):2390-402.doi: 10.1016/j.bbamem.2011.06.019. Epub 2011 Jul 13. PubMed PMID: 21777566.

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Categories
Parameters
None