Coding

Part:BBa_K2660006

Designed by: Victor Nunes de Jesus, Danielle Biscaro Pedrolli   Group: iGEM18_Unesp_Brazil   (2018-10-08)
Revision as of 13:29, 14 October 2018 by VictorNJ (Talk | contribs)


N-Cas9

Coding sequence for the N-terminal section of split Cas9 nuclease.


This BioBrick was engineered to be a split domain of cas9 (BBa_K2457001) coding sequence from Streptococcus pyogenes. It was designed to be an inactive domain linked with proteins like pMag (BBa_K2660009) to control the kinetics of activation by blue light

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
//chassis/prokaryote/bsubtilis
//collections/probiotics/biocontainment
//function/crispr/cas9
Parameters
None