Part:BBa_K2708003
Promoter and S-adenosyl methionine riboswitch, GFP fluorescent expression system.
Characterizing Part:
Here's a map of its gene sequencing.
The circuit consists of the promoter, the S-adenosyl methionine riboswitch and promoter E0840.The plasmid is converted into E.coli to measure green fluorescence to verify the riboswitch's function.When the circuit is activated by the promoter, the terminating substructure at the end of the SAM ribose switch cannot be formed, making the circuit express downward to produce GFP fluorescent protein.While the molecule SAM binds to the SAM riboswitch, the end of the SAM riboswitch forms a terminating substructure that terminates the circuit and fails to express the fluorescent protein.
Its size was 1176bp, and after adding the enzyme cutting sites at both ends, it was between 1500bp and 2000bp. Agarose gel electrophoresis showed the bands correctly.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 963
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