Composite

Part:BBa_K2637033:Design

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 10:43, 14 October 2018 by GangGang (Talk | contribs) (→‎Design Notes)


CikA cassette with TEF1 Promoter and TEF1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 644
    Illegal BamHI site found at 2704
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 266


Design Notes

TEF1 Promoter + CikA + TEF1 Terminator. And CikA has been optimized in yeast.

Source

46456456

References