Regulatory

Part:BBa_K2708002

Designed by: LAN FEI   Group: iGEM18_BIT   (2018-10-02)
Revision as of 10:00, 14 October 2018 by Farfrom (Talk | contribs)


Promoter with an Riboswitch that responds to S-adenosyl methionine molecules.

When the circuit is activated by the promoter, the terminating substructure of the SAM riboswitch cannot be formed, causing the circuit to be expressed downward. When the molecule SAM is combined with the SAM riboswitch, the terminating substructure of the SAM riboswitch is formed to terminate the circuit.

Characterizing Part:

T--BIT--BIT2018 registry-K2708002-1.png

It's made up of promoter BBa_J23100 and the S-adenosyl methionine riboswitch(SAM) BBa_K2708001.When the circuit is activated by the promoter, the terminating substructure of the SAM riboswitch cannot be formed, causing the circuit to be expressed downward. When the molecule SAM is combined with the SAM riboswitch, the terminating substructure of the SAM riboswitch is formed to terminate the circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None