Composite

Part:BBa_K2637030:Design

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 09:32, 14 October 2018 by GangGang (Talk | contribs) (→‎Source)

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KaiA cassette with TEF1 Promoter and TEF1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 266


Design Notes

TEF1 Promoter + KaiA + TEF1 Terminator. And KaiA has been optimized in yeast.

Source

TEF1P and TEF1T are the promoter and terminator of yeast. KaiA is from Synechococcus elongatus PCC7942.

References