Composite

Part:BBa_K2675190:Design

Designed by: Esteban Lebrun   Group: iGEM18_Evry_Paris-Saclay   (2018-10-12)
Revision as of 08:49, 14 October 2018 by ELebrun (Talk | contribs)


sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v1) promote


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 227
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_J23110 constitutive promoter + pAimX(full-noTerminator-v1) promoter (BBa_K2675090) + custom made RBS (BBa_K2675016) + sfGFP-LVAtag coding sequence (BBa_K2675006) + the strong L3S2P56 Terminator (BBa_K2675030)

Source

DNA synthesis & Assembly

References

[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.