Composite

Part:BBa_K2637028:Design

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 06:23, 14 October 2018 by GangGang (Talk | contribs) (→‎Design Notes)


BD-KaiC cassette with TDH3 Promoter and ADH1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 366
    Illegal BglII site found at 1782
    Illegal BglII site found at 2289
    Illegal XhoI site found at 1291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1210


Design Notes

TDH3 Promoter + BD + KaiC + ADH1 Terminator. And KaiC has been optimized in yeast.

Source

45454

References