Composite

Part:BBa_K2637026:Design

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 05:26, 14 October 2018 by GangGang (Talk | contribs) (→‎Design Notes)


BD-CikA cassette with TEF1 Promoter and TEF1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1109
    Illegal BamHI site found at 3169
    Illegal XhoI site found at 753
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 672
    Illegal BsaI.rc site found at 266


Design Notes

TDH3 Promoter + BD + CikA + ADH1 Terminator. And CikA has been optimized in yeast.

Source

46456

References