Composite

Part:BBa_K2637025:Design

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 05:19, 14 October 2018 by GangGang (Talk | contribs) (→‎Source)

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BD-SasA cassette with TDH3 Promoter and ADH1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 366
    Illegal BglII site found at 2058
    Illegal BglII site found at 2127
    Illegal XhoI site found at 1291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1210


Design Notes

TDH3 Promoter + BD + SasA + ADH1 Terminator. And SasA has been optimized in yeast.

Source

TDH3P and ADH1T are the promoter and terminator of yeast. Gal4 DNA binding domain is from yeast genome and SasA is from Synechococcus elongatus PCC7942.

References