Composite

Part:BBa_K2637038

Designed by: Yan Zheng   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 04:59, 14 October 2018 by YJY (Talk | contribs)


Gal1 promoter+NanoLuc+ADH1 terminator

We constructed this cassette which played a prominent role in our measurement process to be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We chose the NanoLuc from the part since it would produce high intensity, glow-type luminescence. Moreover, it also possesses a number of physical properties that make it an excellent reporter protein: small, monomeric enzyme, high thermal stability and so on.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/reporter
//chassis/eukaryote/yeast
//function/reporter/light
Parameters
None