Composite

Part:BBa_K2558204:Design

Designed by: Tsinghua 2018   Group: iGEM18_Tsinghua   (2018-10-05)
Revision as of 03:52, 14 October 2018 by MairZhou (Talk | contribs)

Ptac test device with Anderson medium-expressed lacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1073
    Illegal NheI site found at 1096
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 176


Design Note

In order to investigate how lacI dosage affect IPTG induction we used Anderson promotor J23100, J23110 and J23114 to design three constitutive lacI generator of different intensity. The three lacI generator were then ligated with Ptac controlled reporter sfGFP to make three IPTG induction devices (https://parts.igem.org/Part:BBa_K2558203, https://parts.igem.org/Part:BBa_K2558204, https://parts.igem.org/Part:BBa_K2558205). By measuring sfGFP fluorescence we tested how these devices react to IPTG.


Source

References