Device
dCAS9

Part:BBa_K2841510

Designed by: Jack Lawrence, Rhys Evans, Jonny Whiteside   Group: iGEM18_Warwick   (2018-10-13)
Revision as of 18:13, 13 October 2018 by Psychosahedron (Talk | contribs) (Description)


Streptococcus Pyogenes dCAS9

The dCAS9 protein used in the <a href="https://www.ncbi.nlm.nih.gov/pubmed/23761437">CRISPR repressor 2013 paper</a>. The sequence of the part has been modified to be compatible with iGEM standard. The dCAS9 accepts a <a href="https://parts.igem.org/Part:BBa_K2841514">gRNA</a> which directs it to a site of interest. The <a href="https://www.rcsb.org/structure/4ZT9">structure for the protein</a> family has been solved.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None