Composite

Part:BBa_K2637023:Design

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 14:02, 13 October 2018 by GangGang (Talk | contribs) (→‎Design Notes)


BD-KaiC, Gal4 DNA binding domain fused with KaiC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 725
    Illegal BglII site found at 1232
    Illegal XhoI site found at 234
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 153


Design Notes

Gal4 DNA binding domain fused with KaiC gene. KaiC has been optimized in yeast.

Source

543434

References