Coding

Part:BBa_K2637005:Design

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 06:43, 13 October 2018 by GangGang (Talk | contribs) (→‎Design Notes)


CikA Protein of Circadian Rhythm


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 127
    Illegal BamHI site found at 2187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We performed codon optimization on this gene sequence to use it in yeast. And then we modified some sites to deleted some enzyme cutting sites.

Source

123

References