Part:BBa_K2808002

Invasion protein (invA) gene for the detection of Salmonella typhimurium

The invA gene is involved in the invasion of the cells of the intestinal epithelium and could be involved in the translocation of the InvE protein in Salmonella typhimurium. Due its specificity to the organism and the lack of toxicity, various research projects have used this gene for the detection of S. typhimurium.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1123
  Illegal BglII site found at 1927
  Illegal BamHI site found at 1818
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 602
  Illegal NgoMIV site found at 1113
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1079
  Illegal SapI.rc site found at 1108
  

Experimental Validation

Throughout our project, we successfully ligated our gene invA to the pSB1CR linearized backbone and transformed it into competent DH5-alpha E. coli cells. The part was validated by running a double restriction digestion reaction and PCR on the gene fragment using the respective PCR primers and the amplified regions were obtained as expected. Appropriate bands were seen in the respective lanes, which indicated that the part was successfully ligated and transformed.

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Source

Salmonella typhimurium