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slr0168

Part:BBa_K2559008

Designed by: Yinpin Huang   Group: iGEM18_SCAU-China   (2018-10-12)
Revision as of 03:59, 13 October 2018 by Weiteng (Talk | contribs)


Neutral site for homologous recombination

It allows us to use the culture temperature to regulate the expression of downstream genes

Usage and Biology

The promoter of HSPs is a strong promoter, the amount of the bicistronic, 2.3 kb transcript of groESL1 operon increased 30-fold within 30 min upon heat shock. It allows us to regulate the expression of target genes by changing the culture temperature, and avoid the risk of losing transformed algae strains for toxic effect caused by target genes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1326
    Illegal BamHI site found at 1537
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 776
    Illegal BsaI.rc site found at 1886


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